Coding

Part:BBa_K2785012:Design

Designed by: Zemeng Wei   Group: iGEM18_Pittsburgh   (2018-10-09)


Cytidine Deaminase (CDA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein is originally isolated from the human APOBEC1 protein. It has been codon optimized for Escherichia coli K12 and checked for BioBrick restriction sites using IDT Codon Optimization Tool.

The protein used was from Addgene plasmid pWT037a. This same protein is that used in our experimentation. The part submitted to iGEM has been codon optimized to remove BioBrick sites and has been seqeunce confirmed to be the same amino acid sequence to that used.

Contains a His-6 tag on the 5' end of the protein for purification.


Source

Synthesized by IDT.

References

Navaratnam, N., & Sarwar, R. (2006). An Overview of Cytidine Deaminases. International Journal of Hematology, 83(3), 195-200. doi:10.1532/ijh97.06032

Komor, A. C. (2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 533(7603). doi:10.1038/nature17946

Rewritable multi-event analog recording in bacterial and mammalian cells. Tang W, Liu DR. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. 10.1126/science.aap8992 PubMed 29449507

Liu, D. (2018). PWT037a (Plasmid #107896). Retrieved from https://www.addgene.org/107896/